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KMID : 1149220160240040259
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Korean Journal of Oriental Medical Prescription
2016 Volume.24 No. 4 p.259 ~ p.269
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Anti-inflammatory Effects of the Water Extract of Phyllostachys Folium via NF-κB Inhibition
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Son Jin-Won
Park Sang-Mi
Jung Ji-Yun
Hwangbo Min
Cho Il-Je
Jung Tae-Young
Park Chung-A
Kim Sang-Chan
Jee Seon-Young
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Abstract
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Objectives : Phyllostachys Folium is leaves of Phyllostachys nigra var. henesis STAPF. In the East Asian traditional medicine, the herb has been used to treat nasal bleeding, dysuria, epilepsy and etc. The present study was conducted to evaluate the anti-inflammatory effects of the Phyllostachys Folium water extracts (PFE) in vitro and in vivo model.
Methods : Cell viability was measured by MTT assay after the treatment of PFE and NO production was monitored by measuring the nitrite content in culture medium. iNOS, COX-2, IκB, p-IκBα amd NFκB were detected by immunoblot analysis, and levels of cytokine were analyzed by sandwich ELISA kit. Anti-edema effect of PFE was determined in the carrageenan-induced paw edema model in rats.
Results : LPS increased NO and cytokines levels compared with control, these increases were attenuated by PFE. In addition, LPS-induced pro-inflammatory proteins such as iNOS, COX-2 were down regulated by PFE. These anti-inflammatory effect of PFE results from inhibition of phosphorylation of IκB and translocation of NF-κB.
Conclusion : These results show that PFE has some anti-inflammatory effects which might play a role in gram-negative
bacterial infection inflammation and NFκB activated diseases.
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KEYWORD
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Phyllostachys Folium , Anti-inflammatory effects , NF-κB
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